This course will build on molecular engineering fundaments introduced in ENGS 58 and equip students to formulate novel engineered molecules by translating methods into practical design proposals. The three components of any protein engineering effort will be surveyed: host strain, library design, and selective pressure. Both gold standard and novel engineering methodologies will be studied, and tradeoffs among different techniques will be examined through detailed case studies. Data presentation and interpretation skills will be developed by examining current literature focused on proteins with practical utility.
ENGS 58, OR ENGS 160, OR BIOCHEM 101. Equivalent courses accepted with instructor’s permission.