Research Interests

Metabolic engineering; cellulosic biofuel; metabolic flux analysis; genome resequencing analysis

Education

• BA, Physics, Dartmouth 2004
• BE, Engineering Sciences, Dartmouth 2006
• PhD, Engineering Sciences, Dartmouth 2011

Professional Activities

• Metabolic Engineering Task Leader, DOE Center for Bioenergy Innovation
• Biotechnology Focus Area Leader, Advanced Second Generation Biofuel (A2G) Laboratory, University of Campinas, Brazil
• Metabolic Engineering Group Leader, Lynd Lab

Research Projects

• Genetic tools for anaerobic thermophilic bacteria

  Genetic tools for anaerobic thermophilic bacteria

  In large-scale industrial fermentations, it can be expensive to add oxygen, and to cool fermenters to mesophilic temperatures (20-45C). Using anaerobic thermophilic bacteria avoids both problems, however many genetic tools that have been originally developed for model organisms such as Saccharomyces cerevisiae and Escherichia coli do not work in these organisms. My group is working to develop several types of genetic tools necessary for domestication of thermophilic anaerobic bacteria, including:

  • Ways to get foreign DNA into cells
  • Tightly-controlled inducible promoters for reliable temporal control of gene expression
  • Plasmid-based gene expression systems
  • Chromosomal editing tools
  • Ways to control the mutation rate

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• Metabolic pathway characterization and development

  Metabolic pathway characterization and development

  Metabolism can be understood at many levels of aggregation from individual enzymes to the whole organism. An important intermediate level of aggregation is the metabolic pathway. Developing pathways that enable rapid production of desired compounds at high yield and titer requires a detailed understanding of both the individual components and the systems-level behavior that results from the interaction of these components, including:

  • Identification of constituent enzymes in a pathway and the stoichiometry of the reactions they mediate
  • Characterization of enzyme inhibition and regulation
  • Development of screens and selections to improve properties of key enzymes.
  • Protein engineering to increase activity, decrease inhibition, or change substrate specificity

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• Physiology of native biomass-fermenting organisms

  Physiology of native biomass-fermenting organisms

  Currently, most ethanol is produced using various strains of yeast. These organisms are very good at producing ethanol but have no native ability to consume lignocellulose. This has made it difficult to develop cost-effective yeast-based processes for lignocellulosic ethanol production. My group takes an alternative approach of starting with organisms that natively ferment lignocellulosic biomass and engineering them for efficient biofuel formation. To do this, we need to understand key aspects of the physiology of these native biomass-fermenting organisms, including:

  • Which substrates they can consume, and how these substrates are used for growth, energy production, and product formation.
  • Factors that limit growth and fermentation.
  • Genetic adaptation to stresses associated with industrial fermentation

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Selected Publications

Complete Works and Publications

Courses

• ENGS 161: Metabolic Engineering

News

Biofuels Grant: Energy Technologies
Dec 01, 2021

Dartmouth Engineering Professor to Lead $1.2M DOE Biofuels Grant
Sep 08, 2021

In the News