Lynd Research Lab: Microbial Cellulose Utilization

The Lynd Lab has a long-standing focus on fundamental and applied aspects of microbial cellulose utilization, including aspects of microbial physiology, kinetics, bioenergetics, microbial ecology, and robustness under process-relevant conditions.

Ten most recent papers:

  • Dykstra, A.B., L. St Brice, M. Rodriguez Jr., B. Raman, J. Izquierdo, K.D. Cook, L.R. Lynd, R.L. Hettich. 2014. Development of a multipoint quantitation method to simultaneously measure enzymatic and structural components of the Clostridium thermocellum cellulosome protein complex. J Proteome Res. 13(2):692-701. (Abstract)
  • Izquierdo, J.A., S. Pattathil, A. Guseva, M.G. Hahn, L.R. Lynd. 2014. Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellum to utilize hemicellulose and unpretreated plant material. Biotechnol. Biofuels, 7:136. (Abstract)
  • Holwerda, E.K., P.G. Thorne, D.G. Olson, D. Amador-Noguez, N.L. Engle, T.J. Tschaplinski, J.P. van Dijken, L.R. Lynd. 2014. The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading. Biotechnol. Biofuels. 7:155. (Article)
  • Currie, D.H., A.M. Guss, C.D. Herring, R.J. Giannone, C.M. Johnson, P.K. Lankford, S.D. Brown, R.L. Hettich, L.R. Lynd. 2014. Profile of Secreted Hydrolases, Associated Proteins, and SlpA in Thermoanaerobacterium saccharolyticum during the Degradation of Hemicellulose. Appl. Environ. Microbiol. 80:5001-5011. (Abstract)
  • Reed, P.T., J.A. Izquierdo, L.R. Lynd. 2014. Cellulose fermentation by Clostridium thermocellum and a mixed consortium in an automated repetitive batch reactor. Bioresour. Technol. 155:50-56. (Article)
  • St Brice, L.A., X. Shao, J.A. Izquierdo, L.R. Lynd. 2014. Optimization of affinity digestion for the isolation of cellulosomes from Clostridium thermocellum. Prep. Biochem. Biotech. 44(2):206-216. (Abstract)
  • Dumitrache, A., G.M. Wolfaardt, D.G. Allen, S.N. Liss, L.R. Lynd. 2013. Tracking the cellulolytic activity of Clostridium thermocellum biofilms. Biotech. Biofuels. 6:175-187. (Article)
  • Holwerda, E.K., L.R. Lynd. 2013. Testing alternative kinetic models for utilization of crystalline cellulose (Avicel) by batch cultures of Clostridium thermocellum. Biotechnol. Bioeng. 110(9):2389-2394. (Abstract)
  • Holwerda, E.K., L.D. Ellis, L.R. Lynd. 2013. Development and evaluation of methods to infer biosynthesis and substrate consumption in cultures of cellulolytic microorganisms. Biotechnol. Bioeng. 110(9):2380-2388. (Abstract)
  • Olson, D.G., R.J. Giannone, R.L. Hettich, L.R. Lynd. 2013. Role of the CipA scaffoldin protein in cellulose solubilization, as determined by targeted gene deletion and complementation in Clostridium thermocellum. J. Bacteriol. 195(4):733-739. (Abstract)

Selected additional papers:

  • Waller, B.H., D.G. Olson, D.H. Currie, A.M. Gu, L.R. Lynd. 2013. Exchange of type II dockerin-containing subunits of the Clostridium thermocellum cellulosome as revealed by SNAP-tags. FEMS Microbiol. Lett. 338(1):46-53. (Abstract)
  • Zhou, J., D.G. Olson, D.A. Argyros, Y. Deng, W.M. van Gulik, J.P. van Dijken, L.R. Lynd. 2013. Atypical glycolysis in Clostridium thermocellum. Appl. Environ. Microbiol. 79(9):3000-3008. (Abstract)
  • Podkaminer, K.K., A.M. Guss, H.L. Trajano, D.A. Hogsett, L.R. Lynd. 2012. Characterization of xylan utilization and discovery of a new endoxylanase in Thermoanaerobacterium saccharolyticum through targeted gene deletions. Appl. Environ. Microbiol. 78(23):8441-8447. (Abstract)
  • Mearls, E.B., J.A. Izquierdo and L.R. Lynd. 2012. Formation and characterization of non-growth states in Clostridium thermocellum: spores and L-forms. BMC Microbiol. 12:180. (Abstract)
  • Izquierdo, J.A., L. Goodwin, K.W. Davenport, H. Teshima, D. Bruce, C. Detter, R. Tapia, S. Han, M. Land, L. Hauser, C.D. Jeffries, J. Han, S. Pitluck, M. Nolan, A. Chen, M. Huntemann, K. Mavromatis, N. Mikhailova, K. Liolios, T. Woyke, L.R. Lynd. 2012. Complete genome sequence of Clostridium clariflavum DSM 19732. Stand Genomic Sci. 6(1):104-115. (Abstract)
  • Dumitrache, A., G. Wolfaardt, G. Allen, S.N. Liss and L.R. Lynd. 2012. Form and Function of Clostridium thermocellum Biofilms. Appl. Environ. Microbiol. 79(1):231-239. (Abstract)
  • Ellis, L.D., E.K. Holwerda, D. Hogsett, S. Rogers, X. Shao, T. Tschaplinski, P. Thorne and L.R. Lynd. 2011. Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405). Bioresource Technol. 103(1):293-299. (Article)
  • Sizova, M.V., J.A. Izquierdo, N.S. Panikov, L.R. Lynd. 2011. Cellulose- and xylan-degrading thermophilic anaerobic bacteria from biocompost. Appl. Environ. Microbiol. 77:2282-2291. (Abstract)
  • Olson, D.G., S.A. Tripathi, R.J. Giannone, J. Lo, N.C. Caiazza, D.A. Hogsett, R.L. Hettich, A.M. Guss, G. Dubrovsky, L.R. Lynd. 2010. Deletion of the Cel48S cellulase from Clostridium thermocellum. PNAS. 107(41):17727-17732. (Abstract)
  • Izquierdo, J.A., M.V. Sizova, L.R. Lynd. 2010. Diversity of bacteria and glycosyl hydrolase family 48 genes in cellulolytic consortia enriched from thermophilic biocompost. Appl. Environ. Microbiol. 76(11):3545-3553. (Abstract)
  • Lu, Y., Y.-H.P. Zhang, L.R. Lynd. 2006. Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum. PNAS. 103(44):16165-16169. (Abstract)
  • Zhang, Y.-H.P., L.R. Lynd. 2005. Cellulose utilization by Clostridium thermocellum: Bioenergetics and hydrolysis product assimilation. PNAS. 102:7321-7325. (Abstract)
  • Lynd, L.R., P.J. Weimer, W.H. van Zyl, I.S. Pretorius. 2002. Microbial cellulose utilization: Fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66:506-577. (Abstract)