Lynd Research Lab: Microbial Cellulose Utilization

The Lynd Lab has a long-standing focus on fundamental and applied aspects of microbial cellulose utilization, including aspects of microbial physiology, kinetics, bioenergetics, microbial ecology, and robustness under process-relevant conditions.

Ten most recent papers:

  • Xu Q, Resch MG, Podkaminer K, Yang S, Baker JO, Donohoe BS, Wilson C, Klingeman DM, Olson DG, Decker SR, Giannone RJ, Hettich RL, Brown SD, Lynd LR, Bayer EA, Himmel ME, Bomble YJ. Dramatic performance of Clostridium thermocellum explained by its wide range of cellulase modalities. Sci Adv. 2016 Feb 5;2(2):e1501254. doi: 10.1126/sciadv.1501254. eCollection 2016 Feb. PubMed PMID: 26989779; PubMed Central PMCID: PMC4788478.
  • Herring CD, Thorne PG, Lynd LR. Clostridium thermocellum releases coumaric acid during degradation of untreated grasses by the action of an unknown enzyme. Appl Microbiol Biotechnol. 2016 Mar;100(6):2907-15. doi: 10.1007/s00253-016-7294-1. Epub 2016 Jan 14. PubMed PMID: 26762388.
  • Paye JM, Guseva A, Hammer SK, Gjersing E, Davis MF, Davison BH, Olstad J, Donohoe BS, Nguyen TY, Wyman CE, Pattathil S, Hahn MG, Lynd LR. Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment. Biotechnol Biofuels. 2016 Jan 12;9:8. doi: 10.1186/s13068-015-0412-y. eCollection 2016. PubMed PMID: 26759604; PubMed Central PMCID: PMC4709877.
  • Sand A, Holwerda EK, Ruppertsberger NM, Maloney M, Olson DG, Nataf Y, Borovok I, Sonenshein AL, Bayer EA, Lamed R, Lynd LR, Shoham Y. Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA (σ(A)) and alternative SigI6 (σ(I6)) factors. FEBS Lett. 2015 Oct 7;589(20 Pt B):3133-40. doi: 10.1016/j.febslet.2015.08.026. Epub 2015 Aug 29. PubMed PMID: 26320414.
  • Rooney EA, Rowe KT, Guseva A, Huntemann M, Han JK, Chen A, Kyrpides NC, Mavromatis K, Markowitz VM, Palaniappan K, Ivanova N, Pati A, Liolios K, Nordberg HP, Cantor MN, Hua SX, Shapiro N, Woyke T, Lynd LR, Izquierdo JA. Draft Genome Sequence of the Cellulolytic and Xylanolytic Thermophile Clostridium clariflavum Strain 4-2a. Genome Announc. 2015 Jul 23;3(4). pii: e00797-15. doi: 10.1128/genomeA.00797-15. PubMed PMID: 26205857; PubMed Central PMCID: PMC4513151.
  • Dykstra, A.B., L. St Brice, M. Rodriguez Jr., B. Raman, J. Izquierdo, K.D. Cook, L.R. Lynd, R.L. Hettich. 2014. Development of a multipoint quantitation method to simultaneously measure enzymatic and structural components of the Clostridium thermocellum cellulosome protein complex. J Proteome Res. 13(2):692-701. (Abstract)
  • Izquierdo, J.A., S. Pattathil, A. Guseva, M.G. Hahn, L.R. Lynd. 2014. Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellum to utilize hemicellulose and unpretreated plant material. Biotechnol. Biofuels, 7:136. (Abstract)
  • Holwerda, E.K., P.G. Thorne, D.G. Olson, D. Amador-Noguez, N.L. Engle, T.J. Tschaplinski, J.P. van Dijken, L.R. Lynd. 2014. The exometabolome of Clostridium thermocellum reveals overflow metabolism at high cellulose loading. Biotechnol. Biofuels. 7:155. (Article)
  • Currie, D.H., A.M. Guss, C.D. Herring, R.J. Giannone, C.M. Johnson, P.K. Lankford, S.D. Brown, R.L. Hettich, L.R. Lynd. 2014. Profile of Secreted Hydrolases, Associated Proteins, and SlpA in Thermoanaerobacterium saccharolyticum during the Degradation of Hemicellulose. Appl. Environ. Microbiol. 80:5001-5011. (Abstract)
  • Reed, P.T., J.A. Izquierdo, L.R. Lynd. 2014. Cellulose fermentation by Clostridium thermocellum and a mixed consortium in an automated repetitive batch reactor. Bioresour. Technol. 155:50-56. (Article)

Additional papers:

  • St Brice, L.A., X. Shao, J.A. Izquierdo, L.R. Lynd. 2014. Optimization of affinity digestion for the isolation of cellulosomes from Clostridium thermocellum. Prep. Biochem. Biotech. 44(2):206-216. (Abstract)
  • Dumitrache, A., G.M. Wolfaardt, D.G. Allen, S.N. Liss, L.R. Lynd. 2013. Tracking the cellulolytic activity of Clostridium thermocellum biofilms. Biotech. Biofuels. 6:175-187. (Article)
  • Holwerda, E.K., L.R. Lynd. 2013. Testing alternative kinetic models for utilization of crystalline cellulose (Avicel) by batch cultures of Clostridium thermocellum. Biotechnol. Bioeng. 110(9):2389-2394. (Abstract)
  • Holwerda, E.K., L.D. Ellis, L.R. Lynd. 2013. Development and evaluation of methods to infer biosynthesis and substrate consumption in cultures of cellulolytic microorganisms. Biotechnol. Bioeng. 110(9):2380-2388. (Abstract)
  • Olson, D.G., R.J. Giannone, R.L. Hettich, L.R. Lynd. 2013. Role of the CipA scaffoldin protein in cellulose solubilization, as determined by targeted gene deletion and complementation in Clostridium thermocellum. J. Bacteriol. 195(4):733-739. (Abstract)
  • Waller, B.H., D.G. Olson, D.H. Currie, A.M. Gu, L.R. Lynd. 2013. Exchange of type II dockerin-containing subunits of the Clostridium thermocellum cellulosome as revealed by SNAP-tags. FEMS Microbiol. Lett. 338(1):46-53. (Abstract)
  • Zhou, J., D.G. Olson, D.A. Argyros, Y. Deng, W.M. van Gulik, J.P. van Dijken, L.R. Lynd. 2013. Atypical glycolysis in Clostridium thermocellum. Appl. Environ. Microbiol. 79(9):3000-3008. (Abstract)
  • Podkaminer, K.K., A.M. Guss, H.L. Trajano, D.A. Hogsett, L.R. Lynd. 2012. Characterization of xylan utilization and discovery of a new endoxylanase in Thermoanaerobacterium saccharolyticum through targeted gene deletions. Appl. Environ. Microbiol. 78(23):8441-8447. (Abstract)
  • Mearls, E.B., J.A. Izquierdo and L.R. Lynd. 2012. Formation and characterization of non-growth states in Clostridium thermocellum: spores and L-forms. BMC Microbiol. 12:180. (Abstract)
  • Izquierdo, J.A., L. Goodwin, K.W. Davenport, H. Teshima, D. Bruce, C. Detter, R. Tapia, S. Han, M. Land, L. Hauser, C.D. Jeffries, J. Han, S. Pitluck, M. Nolan, A. Chen, M. Huntemann, K. Mavromatis, N. Mikhailova, K. Liolios, T. Woyke, L.R. Lynd. 2012. Complete genome sequence of Clostridium clariflavum DSM 19732. Stand Genomic Sci. 6(1):104-115. (Abstract)
  • Dumitrache, A., G. Wolfaardt, G. Allen, S.N. Liss and L.R. Lynd. 2012. Form and Function of Clostridium thermocellum Biofilms. Appl. Environ. Microbiol. 79(1):231-239. (Abstract)
  • Ellis, L.D., E.K. Holwerda, D. Hogsett, S. Rogers, X. Shao, T. Tschaplinski, P. Thorne and L.R. Lynd. 2011. Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405). Bioresource Technol. 103(1):293-299. (Article)
  • Sizova, M.V., J.A. Izquierdo, N.S. Panikov, L.R. Lynd. 2011. Cellulose- and xylan-degrading thermophilic anaerobic bacteria from biocompost. Appl. Environ. Microbiol. 77:2282-2291. (Abstract)
  • Olson, D.G., S.A. Tripathi, R.J. Giannone, J. Lo, N.C. Caiazza, D.A. Hogsett, R.L. Hettich, A.M. Guss, G. Dubrovsky, L.R. Lynd. 2010. Deletion of the Cel48S cellulase from Clostridium thermocellum. PNAS. 107(41):17727-17732. (Abstract)
  • Izquierdo, J.A., M.V. Sizova, L.R. Lynd. 2010. Diversity of bacteria and glycosyl hydrolase family 48 genes in cellulolytic consortia enriched from thermophilic biocompost. Appl. Environ. Microbiol. 76(11):3545-3553. (Abstract)
  • Lu, Y., Y.-H.P. Zhang, L.R. Lynd. 2006. Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum. PNAS. 103(44):16165-16169. (Abstract)
  • Zhang, Y.-H.P., L.R. Lynd. 2005. Cellulose utilization by Clostridium thermocellum: Bioenergetics and hydrolysis product assimilation. PNAS. 102:7321-7325. (Abstract)
  • Lynd, L.R., P.J. Weimer, W.H. van Zyl, I.S. Pretorius. 2002. Microbial cellulose utilization: Fundamentals and biotechnology. Microbiol. Mol. Biol. Rev. 66:506-577. (Abstract)