Lynd Research Lab: People | Evert K. Holwerda

Evert K. Holwerda

Evert K. Holwerda

Research Scientist
Thayer School of Engineering
Dartmouth College

Group Leader
Fermentation Engineering
Lynd Lab

evert.k.holwerda.th@dartmouth.edu

Education

  • MSc, Bioprocess Technology, Wageningen University and Research 2006
  • PhD, Engineering Sciences, Dartmouth 2013

Research Interests

  • Solubilization and fermentation of lignocellulosic substrates.
  • Physiology of thermophilic anaerobic cellulolytic bacteria.
  • Optimization of data generation, collection and processing.
  • Cultivation techniques for microorganisms, and technologies that increase lignocellulosic substrate utilization.

Selected Publications

  1. Tian, L., B. Papanek, D.G. Olson, T. Rydzak, E.K. Holwerda, T. Zheng,  J. Zhou, M. Maloney,  N. Jiang,  R.J. Giannone, R.L. Hettich, A.M. Guss, L.R. Lynd (2016). Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum. Biotechnology for Biofuels. 9(116).
  2. Beri D., D.G. Olson, E.K. Holwerda, L.R. Lynd (2016). Nicotinamide cofactor ratios in engineered strains of Clostridium thermocellum and Thermoanaerobacterium saccharolyticum. FEMS microbiology letters. 363 (11).
  3. Sand* A., E.K. Holwerda*, N.M. Ruppertsberger, M. Maloney, D.G. Olson, Y. Nataf, I. Borovok, A.L. Sonenshein, E.A. Bayer, R. Lamed, L.R. Lynd and Y. Shoham (2015). Three cellulosomal xylanase genes in Clostridium thermocellum are regulated by both vegetative SigA and alternative SigI6 factors. FEBS letters. 589(20-B):3111-3140. 
  4. Holwerda, E.K., P.G. Thorne, D.G. Olson, D. Amador-Noguez, N.L. Engle, T.J. Tschaplinski J.P. van Dijken and L.R. Lynd (2014). The exometabolome of Clostridium thermocellumreveals overflow metabolism at high cellulose loading. Biotechnology for Biofuels. 7(155).
  5. Holwerda, E.K. and L.R. Lynd (2013). Testing alternative kinetic models for utilization of crystalline cellulose (Avicel) by batch cultures of Clostridium thermocellum. Biotechnology and Bioengineering. 110(9):2389-2394.
  6. Holwerda, E.K., L.D. Ellis, L.R. Lynd (2013). Development and Evaluation of Methods to Infer Biosynthesis and Substrate Consumption in Cultures of Cellulolytic Microorganisms. Biotechnology and Bioengineering. 110(9):2380-2388.
  7. Holwerda, E.K., K.D. Hirst and L.R. Lynd (2012). A defined growth medium with very low background carbon for culturing Clostridium thermocellum. Journal of Industrial Microbiology and Biotechnology. 39(6):943-947.
  8. Ellis, L.D., E.K. Holwerda, D. Hogsett, S. Rogers, X. Shao, T. Tschaplinski, P. Thorne and L.R. Lynd (2011). Closing the carbon balance for fermentation by Clostridium thermocellum (ATCC 27405). Bioresource Technology. 103(1):293-299.