Griswold Group: Publications


Peer Reviewed


  • Salvat R, Moise L, Bailey-Kellogg C, Griswold KE (2014) A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes. Journal of visualized experiments : JoVE (85). doi:10.3791/51308 [Pubmed]
  • Griswold KE, Bement JL, Teneback CC, Scanlon TC, Wargo MJ, Leclair LW (2014) Bioengineered lysozyme in combination therapies for Pseudomonas aeruginosa lung infections. Bioengineered 5 (2):(epub ahead of print) [Pubmed]
  • Zhao H, Blazanovic K, Choi Y, Bailey-Kellogg C, Griswold KE (2014) Gene and protein sequence optimization for high level production of fully active and aglycosylated lysostaphin in Pichia pastoris. Applied and Environmental Microbiology. doi:10.1128/aem.03914-13 [Pubmed]


  • Teneback C.C., Scanlon T.C., Wargo M..J, Bement J.L., Griswold K.E., Leclair L.W.  Bioengineered lysozyme reduces bacterial burden and inflammation in a murine model of mucoid Pseudomonas aeruginosa lung infection.  Antimicrobial Agents and Chemotherapy (2013), 57(11), pp. 5559-5564 [Pubmed]
  • Scanlon T.C., Dostal, S.M., Griswold, K.E.  A High Throughput Screen for Antibiotic Drug Discovery. Biotechnology & Bioengineering (2013), [Epub ahead of print]; PMID: 23955804 [Pubmed]
  • Lamppa J.W., Tanyos S.A., Griswold K.E.  Engineering Escherichia coli for Soluble Expression and Single Step Purification of Active Human Lysozyme.  Journal of Biotechnology (2013), 164, pp. 1-8 [Pubmed]
  • Choi Y., Griswold K.E., Bailey-Kellogg C.  Structure-based Redesign of Proteins for Minimal T cell Epitope Content.  Journal of Computational Chemistry (2013), 34(10), pp. 879-891 [Pubmed]
  • Lamppa J.W. and Griswold K.E.  Alginate Lyase Exhibits Catalysis-Independent Biofilm Dispersion and Antibiotic Synergy.  Antimicrobial Agents and Chemotherapy (2013), 57(1), pp. 137-145 [Pubmed]
  • Parker A.S., Choi Y.J., Griswold K.E., Bailey-Kellogg C. Structure-Guided Deimmunization of Therapeutic Proteins. Journal of Computational Biology (2013), 20(2), pp. 152-165 [Pubmed]


  • Osipovitch D.C., Parker A.S., Makokha C.D., Desrosiers J., Kett W.C., Moise L., Bailey-Kellogg C., Griswold K.E. Design and Analysis of Immune-Evading Enzymes for ADEPT Therapy. Protein Engineering, Design & Selection (2012), 25(10), pp. 613-624 [Pubmed]


  • Parker, A.S., Griswold, K.E., Bailey-Kellogg, C. Optimization of combinatorial mutagenesis. Journal of Computational Biology (2011), 18(11), pp. 1-14 [Pubmed]
  • Parker, A.S., Griswold, K.E., Bailey-Kellogg, C. Optimization of Therapeutic Proteins to Delete T-Cell Epitopes While Maintaining Beneficial Residue Interactions. Journal of Bioinformatics and Computational Biology (2011), 9(2), pp. 207-229 [Pubmed]
  • Lamppa, J.W., Ackerman, M.E., Lai, J.I., Scanlon, T.C., Griswold, K.E. Genetically Engineered Alginate Lyase-PEG Conjugates Exhibit Enhanced Catalytic Function and Reduced Immunoreactivity. PLoS One (2011), 6(2): e17042 [Pubmed]
  • Gill, A., Scanlon, T.C., Osipovitch, D.C., Madden, D.R., Griswold, K.E. Crystal structure of a charge engineered human lysozyme having enhanced bactericidal activity. PLoS One (2011), 6(3): e16788 [Pubmed]


  • Scanlon, T.C., Teneback, C.C., Gill, A., Bement, J.L., Lamppa, J.W., Weiner, J.A., Leclair, L.W., Griswold, K.E. Enhanced antimicrobial activity of engineered human lysozyme. ACS Chemical Biology (2010), 5(9), pp. 809-818. [Pubmed]
  • Parker, A.S., Zheng, W., Griswold, K.E., Bailey-Kellogg, C. Optimization algorithms for functional deimmunization of therapeutic proteins. BMC Bioinformatics (2010), 11(1), 180. [Pubmed]
  • Zheng, W., Griswold, K.E., Bailey-Kellogg, C. Protein fragment swapping: A method for asymmetric, selective site-directed recombination. Journal of Computational Biology (2010), 17(3), pp. 459-475. [Pubmed]
  • Scanlon, T.C., Jewell, H.L., Gray, E.C., Griswold, K.E. The Monoclonal Myth: addenda to Quantifying and resolving multiple vector transformants in S. cerevisiae plasmid libraries. Bioengineered Bugs, (2010), 1(3), pp. 1-4. [Bioengineered Bugs]


  • Scanlon*, T.C., Gray*†, E.C., Griswold, K.E. Quantifying and Resolving Multiple Vector Transformants in S. cerevisiae Plasmid Libraries. BMC Biotechnology (2009), 9(1), 95. [Pubmed]
  • Zheng, W., Griswold, K.E., Bailey-Kellogg, C. Protein fragment swapping: A method for asymmetric, selective site-directed recombination. Proceedings of the 13th Annual International Conference on Research in Computational Molecular Biology (2009) pp. 321-338. [Link]


  • Griswold, K.E., Aiyappan, N.S., Iverson, B.L., Georgiou, G. The Evolution of Catalytic Efficiency and Substrate Promiscuity in Human Theta Class 1-1 Glutathione Transferase. Journal of Molecular Biology (2006), 364(3), 400-10. [Pubmed]


  • Griswold, K.E., Kawarasaki, Y., Ghoneim, N., Benkovic, S.J., Iverson, B.L., Georgiou, G. Evolution of highly active enzymes by homology-independent recombination. Proceedings of the National Academy of Sciences, USA (2005), 102(29), 10082-7. [Pubmed]
  • Levy, M., Griswold, K.E., Ellington, A.D. Direct selection of trans-acting ligase ribozymes by in-vitro compartmentalization. RNA (2005), 11(10):1555-62. [Pubmed]


  • Kawarasaki, Y., Griswold, K.E., Stevenson, J.D., Selzer, T., Benkovic, S.J., Iverson, B.L., Georgiou, G. Enhanced crossover SCRATCHY: construction and high-throughput screening of a combinatorial library containing multiple non-homologous crossovers. Nucleic Acids Research (2003), 31(21), e126/1-e126/8. [Pubmed]
  • Griswold, K.E., Mahmood, N.A., Iverson, B.L., Georgiou, G. Effects of codon usage versus putative 5'-mRNA structure on the expression of Fusarium solani cutinase in the Escherichia coli cytoplasm. Protein Expression and Purification (2003), 27(1), 134-42. [Pubmed]


  • Griswold, K.E. pH sensing agar plate assays for esterolytic enzyme activity. Methods in Molecular Biology (Totowa, NJ, United States) (2003), 230, 203-11. [Pubmed]

Book Chapters

  • Georgiou, G., Harvey, B.R., Griswold, K.E., Iverson, B.L. (2005). Applications of Flow Cytometry in Protein Engineering. (Chapter 12) In Larry A. Sklar (Ed), Flow Cytometry in Biotechnology. Oxford University Press. [Google Books]